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plasmid characteristics source reference c glutamicum strains delaro5 po6 iolt1 prophage  (ATCC)


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    ATCC plasmid characteristics source reference c glutamicum strains delaro5 po6 iolt1 prophage
    Plasmid Characteristics Source Reference C Glutamicum Strains Delaro5 Po6 Iolt1 Prophage, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 11967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    All plasmids generated in this study

    Journal: Microbial Cell Factories

    Article Title: Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

    doi: 10.1186/s12934-024-02368-3

    Figure Lengend Snippet: All plasmids generated in this study

    Article Snippet: It was kindly provided by Bisy GmbH (Hofstaetten a. d. Raab, Austria) and was made by deletion of the GUT1 gene of the killer plasmid-free Mut S platform strain K. phaffii BG11 (Biogrammatics, Carlsbad, CA).

    Techniques: Generated, Selection, Marker, Clone Assay, Expressing

    Vector map of a general ARS plasmid carrying a carbon marker cassette. Besides an origin of replication and an Ampicillin resistance cassette for cloning in E. coli , the episomal plasmids used in this study harbor the PARS1 sequence for the replication in K. phaffii , a reporter cassette with the de-repressable P DC promoter driving Ca lB expression, whose secretion is facilitated by the D-alpha signal sequence, a deletion variant of the S. cerevisiae mating factor alpha signal peptide. Selective pressure in K. phaffii is provided by either GUT1 or TPI1 genes with one of the 5 tested promoters placed upstream

    Journal: Microbial Cell Factories

    Article Title: Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

    doi: 10.1186/s12934-024-02368-3

    Figure Lengend Snippet: Vector map of a general ARS plasmid carrying a carbon marker cassette. Besides an origin of replication and an Ampicillin resistance cassette for cloning in E. coli , the episomal plasmids used in this study harbor the PARS1 sequence for the replication in K. phaffii , a reporter cassette with the de-repressable P DC promoter driving Ca lB expression, whose secretion is facilitated by the D-alpha signal sequence, a deletion variant of the S. cerevisiae mating factor alpha signal peptide. Selective pressure in K. phaffii is provided by either GUT1 or TPI1 genes with one of the 5 tested promoters placed upstream

    Article Snippet: It was kindly provided by Bisy GmbH (Hofstaetten a. d. Raab, Austria) and was made by deletion of the GUT1 gene of the killer plasmid-free Mut S platform strain K. phaffii BG11 (Biogrammatics, Carlsbad, CA).

    Techniques: Plasmid Preparation, Marker, Clone Assay, Sequencing, Expressing, Variant Assay